289: MinION detection of chimeric reads in murine Ifna/Ifnb amplicons and ligation-related artifact prevalence

White R et al., F1000Research - Investigation of chimeric reads in MinION nanopore sequencing of short PCR amplicons, focusing on ligation-related artifacts, barcode tracing, and the prevalence of cross-gene chimeras in murine Ifna/Ifnb sequencing runs.

Music:
Enjoy the music based on this article at the end of the episode.

Article title:
Investigation of chimeric reads using the MinION

First author:
White R

Journal:
F1000Research

DOI:
10.12688/f1000research.11547.2

Reference:
White R, Pellefigues C, Ronchese F, Lamiable O, Eccles D. Investigation of chimeric reads using the MinION [version 2; peer review: 2 approved]. F1000Research. 2017;6:631. https://doi.org/10.12688/f1000research.11547.2

License:
This episode is based on an open-access article published under the Creative Commons Attribution 4.0 International License (CC BY 4.0) – https://creativecommons.org/licenses/by/4.0/

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Official website https://basebybase.com

On PaperCast Base by Base you’ll discover the latest in genomics, functional genomics, structural genomics, and proteomics.

QC:
This episode was reviewed as a special editorial release.

QC Scope:
- human-authored narration, music, and publication metadata
- excludes automated single-article AI QC because this episode was not produced by the standard AI narration pipeline
- source attribution and descriptive metadata were reviewed manually

QC Summary:
- production mode: human-written and human-produced episode
- automated transcript-vs-paper AI QC: not applicable
- metadata, source attribution, and release assets were reviewed manually

Metadata Audited:
- article_doi
- article_title
- article_journal
- reference
- license

Factual Items Audited:
- episode classified as a human-produced release
- canonical human narration retained as the source of record
- canonical human-produced music retained as the source of record
- automated NotebookLM regeneration intentionally not used for this episode

QC result: Editorial exception. This episode was approved after manual review.

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