An accidental discovery: From failed experiment to new antibiotic

An accidental discovery: From failed experiment to new antibiotic

NTNU professor Marit Otterlei nearly threw out the contaminated cell culture where she and her colleagues were testing a new cancer drug.

The problem arose on a hot summer day, in Trondheim, in a country not known for hot summer days. So they'd opened the lab's windows overnight.

When they came back the next day, they found an uninvited guest, snuggled in with their cancer cell culture: Bacteria!!!

Here's the thing, though: although the drug had been designed to work on human cancer cells, it looked like it had killed the bacteria, too!

That was remarkable, because the cancer drug targeted a specific mechanism that human cells use to replicate. It looked like the drug also targeted the same mechanism in bacteria -- even though the tree of life had branched away from bacteria 3 BILLION years ago! How could that be?

Today's podcast takes a peek into the challenging world of what it takes to bring a drug, especially an antibiotic, to market. But it’s also an inside look into how some researchers, with their deep curiosity about the nuts and bolts of how life actually works, can come up with startling discoveries that may someday save our lives. Sometimes, the key to saving lives can be hidden in a protein that hasn't changed much over billions of years.

Our guests on today's show are Marit Otterlei, a professor at NTNU's Department of Clinical and Molecular Medicine; Siril Skaret Bakke, innovation manager at NTNU's Technology Transfer Office AS, and Christine Årdal , senior researcher at the Norwegian Institute of Public Health.

Marit is a part-time CSO at APIM Therapeutics (https://www.apimtherapeutics.com/), which is developing the cancer drug that she was testing back in 2011, when an open window on a hot summer day led her to suspect that her substance might a possible antibiotic, too. That antibiotic, Betatide, is now undergoing testing that pharmaceutical companies require before investing in it.

Here's a list of some of the key academic publications:

Gilljam, Karin Margaretha; Feyzi, Emadoldin; Aas, Per Arne; Sousa, Mirta; Müller, Rebekka; Vågbø, Cathrine Broberg. (2009) Identification of a novel, widespread, and functionally important PCNA-binding motif. Journal of Cell Biology

Nedal, Aina; Ræder, Synnøve Brandt; Dalhus, Bjørn; Helgesen, Emily; Forstrøm, Rune Johansen; Lindland, Kim. (2020) Peptides containing the PCNA interacting motif APIM bind to the beta-clamp and inhibit bacterial growth and mutagenesis. Nucleic Acids Research (NAR)

Nepal, Anala; Ræder, Synnøve Brandt; Søgaard, Caroline Krogh; Haugan, Maria Schei; Otterlei, Marit. (2021) Broad-Spectrum Antibacterial Peptide Kills Extracellular and Intracellular Bacteria Without Affecting Epithelialization. Frontiers in Microbiology

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Shedding light — on the polar night

Shedding light — on the polar night

Krill eyeballs. The werewolf effect. Diel vertical migration. Arctic marine biologists really talk about these things. There’s a reason for that — when it comes to the polar night, when humans see only velvety darkness, krill eyeballs see things a little differently. And when the sun has been gone for months, during the darkest periods of the polar night, the moon does unexpected things to marine organisms. Learn more about what biologists are figuring out about the workings of the polar night — and what it means at a time when the Arctic is warming at a breakneck pace. Our guests for this episode were Jørgen Berge, Geir Johnsen, Laura Hobbs and Jonathan H. Cohen. You can see a transcript of the episode here. Fridtjof Nansen’s book about his Arctic expedition is called Farthest North. You can also read about the other influences his pioneering journey had on science here. You can also read about Geir Johnsen’s different research projects in a series of articles from Norwegian SciTech News. The findings of the polar night team are so surprising that they actually wrote a textbook about it, edited by Jørgen Berge, Geir Johnsen and Jonathan H. Cohen. The book is titled Polar Night Marine Ecology: Life and Light in the Dead of Night. Here are some of the scientific articles describing the polar night research: Berge, J., Båtnes, A.S., Johnsen, G. et a. (2012) Bioluminescence in the high Arctic during the polar night. Mar Biol 159: 231-237 Berge, J., Renaud, P. E., Darnis, G. et al. (2015) In the dark: A review of ecosystem processes during the Arctic polar night. Progress in Oceanography, 139: 258-271 Berge, J., Daase, M., Renaud, P.E. et al. (2015) Unexpected Levels of Biological Activity during the Polar Night Offer New Perspectives on a Warming Arctic Current Biology,25, 2555-2561. Cohen J.H., Berge J., Moline M.A. et al. (2015) Is Ambient Light during the High Arctic Polar Night Sufficient to Act as a Visual Cue for Zooplankton? PLoS ONE 10(6): e0126247. doi:10.1371/journal.pone.0126247 Ludvigsen, M., Berge, J., Geoffroy, M. et al. (2018) Use of an Autonomous Surface Vehicle reveals small-scale diel vertical migrations of zooplankton and susceptibility to light pollution under low solar irradiance. Science Advances 4: eaap9887 Hobbs L, Cottier FR, Last KS, Berge J (2018) Pan-Arctic diel vertical migration during the polar night. Mar Ecol Prog Ser 605:61-72. Berge, Jørgen; Geoffroy, Maxime; Daase, Malin; Cottier, Finlo Robert; Priou, Pierre; Cohen, Jonathan H.; Johnsen, Geir; McKee, David; Kostakis, Ina; Renaud, Paul Eric; Vogedes, Daniel Ludwig; Anderson, Philip J.; Last, Kim S.; Gauthier, Stephane. (2020) Artificial light during the polar night disrupts Arctic fish and zooplankton behavior down to 200 m depth. Communications Biology. 3 (102), 10.1038/s42003-020-0807-6 Hosted on Acast. See acast.com/privacy for more information.

27 Jan 202124min

Sneak peak

Sneak peak

Ever wonder what's happening in some of the more far-flung places on the planet? In 63 Degrees North, we'll bring you stories from Norway every week about surprising science, little-known history, and technology and engineering discoveries that can help change the world. The first of five episodes drops February 1. Brought to you by NTNU, the Norwegian University of Science and Technology. Hosted on Acast. See acast.com/privacy for more information.

20 Jan 20212min

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